Running session-conditioned human serum lowers prostate cancer cell spheroid formation.

ABSTRACT:

Purpose

Physical activity is associated with a lower mortality and recurrence risk in cancer, yet the underlying mechanisms remain unclear. This study aimed to evaluate the effects of running sessions on the tumorigenic potential of prostate cancer cells using a 3D in vitro model.

Methods

Fifteen healthy males completed two outdoor running sessions (5 km and 10 km), interspersed by 1 month of wash-out time. Blood samples were collected before (PRE), immediately after (POST), and 3 h after (POST-3 h) sessions. Human serum (HS) samples were used to stimulate LNCaP and PC3 cell lines in 3D in vitro culture technique. The spheroid formation ability was quantified after 21 days of incubation, using GelCount.

Results

In both prostate cancer cell lines, a reduction in sphero

SUBMITTER: Baldelli G 

PROVIDER: S-EPMC12535562 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature