Standardized dynamic glucose testing reveals donor-dependent heterogeneity in human islet function.

ABSTRACT: Glucose-stimulated insulin secretion (GSIS) testing of isolated islets of Langerhans is crucial for assessing β-cell function, yet protocol variability complicates result interpretation. This study investigated insulin secretion heterogeneity across 576 donors and examined the influence of donor characteristics on secretory responses. We compared static incubation (n = 408) and dynamic perifusion (n = 168) techniques using standardized glucose stimulation protocols (3 vs 15 mM). While both methods showed comparable stimulation indices (r² = 0.652), dynamic perifusion uniquely captured temporal secretion patterns and revealed greater dynamic range in insulin responses. Notably, dynamic perifusion, with insulin content normalization, revealed a 22-fold variati

SUBMITTER: Gnanasekar P 

PROVIDER: S-EPMC12535635 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature