Determining the Serum Stability of Human Adenosine Deaminase 1 Enzyme.

ABSTRACT: The concept of enzyme stability is typically used to refer to an enzyme's thermostability - its ability to retain structure and activity as temperature increases. For a therapeutic enzyme, other measures of stability may also be critical, particularly its ability to retain function in human serum at 37 °C, which we refer to as serum stability. Here, we describe an in vitro assay to assess the serum stability of the wildtype Homo sapiens adenosine deaminase I (HsADA1) enzyme using an absorbance-based microplate procedure. Specifically, this manuscript describes the preparation of buffers and reagents, a method arranging for the coincubation of HsADA1 in serum, a method to analyze the test samples using a microplate reader, and an accompanying analysis to determine the fraction of activity t

SUBMITTER: Haikal Y 

PROVIDER: S-EPMC12535683 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature