A Single-Step Protein Extraction for Lung Extracellular Matrix Proteomics Enabled by the Photocleavable Surfactant Azo and timsTOF Pro.

ABSTRACT: The extracellular matrix (ECM) is a dynamic, complex network of proteins, collectively known as the "matrisome," which not only provides essential structural support to cells and tissues but also regulates critical cellular processes. Dysregulation of ECM is implicated in many diseases, underscoring the need to characterize the matrisome to better understand disease mechanisms. We have previously developed a dual-step protocol enabled by the photocleavable surfactant Azo for the extraction of ECM proteins from tissue using pH-neutral decellularization followed by solubilization by Azo. While effective for characterization of ECM proteins, such a dual-step protocol requires two extracts per sample, limiting the throughput and complicating the comparison of protein quantitation across differ

SUBMITTER: Towler AG 

PROVIDER: S-EPMC12535824 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature