Development of a Multiplex TaqMan Real-Time RT-PCR Assay for the Rapid Differential Detection of Classic, MLB-Clade and VA-Clade Human Astroviruses.

ABSTRACT: The classical human astrovirus (HAstV) causes acute or severe gastroenteritis mainly among children and the elderly populations. The novel divergent HAstV-VA and HAstV-MLB clades related to encephalitis in immunocompromised patients, are genetically more similar to certain animal astroviruses, and have the potential for cross-species transmission. To identify all the three HAstV clades in a single reaction, we developed a quantitative reverse transcription PCR (RT-qPCR) method. Primers and probes were designed based on the conserved regions of the HAstV genomes that exhibited inter-clade divergence. This method was highly sensitive, as the detection limits were 95, 12.5 and 25 copies/μl for the classical HAstV, HAstV-MLB and HAstV-VA strains, respectively. Intra- and inter-assay variabilit

SUBMITTER: Zheng P 

PROVIDER: S-EPMC12535854 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature