Development of potent HLA-A02:01-restricted peptide-based cytotoxic T-cells against SARS-CoV-2 infections in patients awaiting a kidney transplant.

ABSTRACT:

Background

Controlling viral infections prior to solid organ transplantation is vital for successful engraftment and overall well-being of patients. One promising approach involves the deployment of viral antigen-specific cytotoxic T cells to eradicate viral pathogens. Although there have been attempts to develop anti-viral vaccines in the literature, the limited number of virus-specific cells which can be generated in vitro in the autologous system make it impracticable for autologous therapy.

Methods

We developed a straightforward and scalable method for the in vitro expansion of SARS-CoV-2 Spike S1 peptide-specific cytotoxic CD8⁺ T cells. This was achieved through combinatorial stimulation with S peptide-conjugated polystyrene microspheres in the

SUBMITTER: Chang CC 

PROVIDER: S-EPMC12535972 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature