Predicting immune checkpoint inhibitors response via fluorescence lifetime imaging microscopy: a systematic review.

ABSTRACT:

Introduction

Fluorescence Lifetime Imaging Microscopy (FLIM) is an imaging technique that allows for the visualization of the cellular microenvironment by measuring the decay time of endogenous fluorescent molecules. Its advent has allowed the acquisition of information on previously undetectable aspects of the tissue environment, which also includes some mechanisms involving immune checkpoints. Understanding the level of interaction with their ligands is of paramount importance when stratifying patients for immunotherapy, as traditional methods such as immunohistochemistry (IHC) were found to be ineffective in predicting responders.

Methods

This review analyzes the current literature on FLIM as a means of predicting targets' responsiveness to ICIs by examining the most rele

SUBMITTER: Cossa C 

PROVIDER: S-EPMC12535979 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature