Characterization of Chronic Lymphocytic Leukemia Immunoglobulin Rearrangements from Partial Read Sequencing.

ABSTRACT: The determination of the mutational status in the immunoglobulin variable region is an established prognostic biomarker for chronic lymphocytic leukemia (CLL). The length and inner variability of the variable, diversity, and joining (VDJ) rearranged sequences compromise B-cell clone characterization using next-generation sequencing (NGS), and a standardization is needed to adapt the procedure to the current clinical guidelines. Here, we develop a complete strategy for sequencing the variable domain of the immunoglobulin heavy chain (IGH) locus with a simple, low-cost, and efficient method that enables sequencing using shorter reads (MiSeq 150 × 2), allowing for faster results. Clonality and mutational status determination are performed within the same analysis pipeline. We tested and valid

SUBMITTER: Fuentes-Trillo A 

PROVIDER: S-EPMC12536063 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature