Blood mononuclear cells induce accelerated vascular remodeling under acute inflammation in vitro.

ABSTRACT: The plasticity of blood mononuclear cells (MCs) and their role in vascular remodeling have been the focus of many studies; however, their in vitro differentiation efficiency remains poorly understood. Herein, we demonstrate that the inflammatory response accelerates the efficiency of MCs differentiation into endothelial-like cells through chemical cues in vitro. RT-PCR and RNA sequencing revealed that the differentiated cells exhibited upregulated pathways associated with vascular remodeling and regeneration. In contrast, MCs collected from normal blood showed a differentiation bias toward macrophages. Notably, under inflammatory conditions, primarily monocytes transitioned into the CD14++/CD16+/CD163+ subset, which contributed significantly to vascular remodeling. This trans

SUBMITTER: Soni R 

PROVIDER: S-EPMC12536093 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature