Verification of Preferred Reference Genes for RT-qPCR Analysis of Radiosensitivity Gene in Human Peripheral Blood.

ABSTRACT:

Objective

High-throughput gene expression analysis represents a promising approach for evaluating irradiation doses in vitro. This study aimed to identify preferred reference genes for normalizing RT-qPCR data in human peripheral blood following X-ray irradiation.

Methods

We assessed the stability of GAPDH, 18S rRNA, UBC, HPRT, DPM1, ITFG1, MRPS5, and ACTB, which are commonly utilized in radiation biodosimetry studies. Three whole blood samples from healthy donors were divided into three culture time groups (2, 12, and 24 hours) and subjected to X-ray doses of 0, 0.5, 1.2, and 3.5 Gy, resulting in a total of 12 subgroups. NormFinder, geNorm, BestKeeper, and ΔCt were employed to evaluate the stability of the candidate ge

SUBMITTER: He M 

PROVIDER: S-EPMC12536175 | biostudies-literature | 2025 Oct-Dec

REPOSITORIES: biostudies-literature