Distinct Immunosuppressive Tumor Microenvironment in Gastric Cancer With Peritoneal Metastasis.

ABSTRACT:

Purpose

Immunotherapy combined with chemotherapy is the standard palliative treatment for gastric cancer. However, peritoneal metastases are often resistant to immunotherapy, underscoring the need to better understand the tumor immune microenvironment (TIME). In this study, we aimed to comprehensively analyze the TIME in peritoneal metastases of gastric cancer.

Materials and methods

Paired single-cell suspensions from malignant ascites and peripheral blood mononuclear cells (PBMCs) were obtained from 27 patients with gastric cancer for multicolor fluorescence-activated cell sorting (FACS) analysis. Cell-free fluids from malignant ascites and plasma of 15 patients with gastric cancer, along with benign ascites from 15 patients with liver cirrhosis, were analyzed using multipl

SUBMITTER: Kim H 

PROVIDER: S-EPMC12536193 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature