Deciphering the regulatory role of ELF5 in buffalo lactation.

ABSTRACT: E74 Like ETS Transcription Factor 5 (ELF5) has been implicated in milk protein synthesis in various mammals, but its precise roles and mechanisms in buffalo have remained largely unknown. This study successfully isolated and characterized ELF5 from buffalo mammary gland tissues, revealing a 768 bp coding sequence (CDS) that translates into a 255 amino acid protein. Bioinformatics analysis identified a conserved ETS domain within ELF5, crucial for transcriptional regulation, along with several predicted post-translational modification sites, including phosphorylation, N-glycosylation, and N-myristoylation. Molecular docking analysis further showed that ELF5 probably interacts with the STAT5A protein through hydrogen bonds and salt bridges, and forms hydrogen bonds with STAT5B, sugges

SUBMITTER: Gao R 

PROVIDER: S-EPMC12536222 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature