PGC7 maintains the pluripotency of F9 embryonic carcinoma cells by promoting Nanog translation.

ABSTRACT: Primordial germ cell 7 (PGC7) is prominently expressed in primordial germ cells (PGCs) and embryonic stem cells (ESCs), serving as a pivotal marker for discerning stem cell pluripotency. However, the role of PGC7 in regulating core pluripotency factors remains unclear. In this study, the expression dynamics of PGC7 and pluripotency-associated proteins are systematically evaluated by quantitative reverse transcription PCR (RT-qPCR) and western blot analysis. Complementary experimental approaches including confocal immunofluorescence and Co-immunoprecipitation (Co-IP) assays are subsequently employed to establish subcellular colocalization patterns and elucidate the molecular mechanisms associated with PGC7 function. The results show that PGC7 is closely associated with the pluripotency stat

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12536462 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature