RSK1 and RSK2 modulate the translatome of glioblastoma cells in an isoform-specific and mTORC1 independent manner.

ABSTRACT:

Background

The p90 ribosomal S6 kinase (RSK) family, downstream target of Ras/ERK signaling, encompasses four human isoforms (RSK1-4). Glioblastomas (GBMs) predominantly express RSK1 and RSK2, whereby RSK1 is markedly upregulated in a subset of GBMs associated with dismal prognosis and immune infiltration, while RSK2 expression is constant. RSKs were proposed as regulators of mRNA translation through the activation of mTORC1 and other factors, such as eIF4B, but nothing is known about their effect on the translatome of GBM cells.

Methods

Through the generation of RSK1 and RSK2 knockout as well as double knockout (DKO) GBM cells, we investigated RSK isoform-specific functions in cell signaling, followed by the identification of their distinct transcriptome and translatome.

SUBMITTER: Roffe M 

PROVIDER: S-EPMC12536492 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature