Hypo-osmolarity promotes naive pluripotency by reshaping cytoskeleton and increasing chromatin accessibility.

ABSTRACT:

Introduction

Cell fate determination and transition are of paramount importance in biology and medicine. Naive pluripotency could be achieved by reprogramming differentiated cells. However, the mechanism is less clear. Osmolarity is an essential physical factor that acts on living cells, especially for pluripotent cells, but its significance in cell fate transition remains unexplored.

Objectives

To investigate the role of osmolarity in cell fate transition and its underlying mechanism.

Methods

Flow cytometry, quantitative real-time PCR, teratoma and chimeric mice assays were performed to assess reprogramming efficiency and characterize iPSCs. TEM, immunofluorescence staining, western blot, chemical treatment and genetic modification were utilized to evaluate cell morp

SUBMITTER: Lu R 

PROVIDER: S-EPMC12536593 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature