SUMOylation modulates the dual functions of Kruppel homolog 1 in transcriptional regulation of Broad-Complex expression.

ABSTRACT:

Introduction

Insects undergo intricate metamorphosis processes governed by hormonal regulatory mechanisms, exemplified by the antagonistic actions of 20-hydroxyecdysone (20E) and juvenile hormone (JH). Krüppel homolog 1 (Kr-h1) is a key transcription factor mediating JH's antagonistic effects on 20E. Previous studies have shown that Kr-h1 plays dual roles in regulating the 20E signaling gene Broad-Complex (Br-C), repressing it in larvae and activating it during pupation. However, the mechanisms underlying this dual functionality remain elusive.

Objectives

This study aimed to determine whether SUMOylation, a post-translational modification, functions as part of a molecular switch mechanism for Kr-h1's stage-specific transcriptional roles.

Methods

Using Drosophila melan

SUBMITTER: He Q 

PROVIDER: S-EPMC12536605 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature