Cross-talk between engineered <i>Clostridium acetobutylicum</i> and <i>Clostridium ljungdahlii</i> in syntrophic cocultures enhances isopropanol and butanol production.

ABSTRACT: There is a need for efficient and sustainable production of essential chemicals such as isopropanol and butanol from renewable sugar feedstocks. Microbial fermentations use glycolysis, and as result, a third of the sugar carbon is lost to CO₂ through pyruvate decarboxylation to acetyl-CoA, the starting intermediate for the biosynthesis of most microbial metabolites. In nature, microbes exist in syntrophic consortia, allowing for mutually-beneficial interactions, the production of novel products, and the realization of novel benefits-including better carbon conservation-not seen in monocultures. We examined the impact of starting coculture cell densities, the gas atmosphere (N₂, H₂, or H₂/CO₂) and coculture species ratios (using a recen

SUBMITTER: Otten JK 

PROVIDER: S-EPMC12536658 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature