Genetic perturbation of cellular homeostasis regulates integrated stress response signaling to control Drosophila hematopoiesis.

ABSTRACT: Aging results in a decline in cellular and molecular functions. One of the hallmarks of aging is stem cell exhaustion, which impacts self-renewal and differentiation. We employ the Drosophila larval lymph gland (LG) to investigate the impact of genetic perturbation of cellular homeostasis on hematopoiesis. The LG consists of a posterior signaling center (PSC) - a stem cell niche that maintains medullary zone (MZ) prohemocytes, whereas the cortical zone (CZ) consists of differentiated hemocytes. We employed over-activation of Toll or Imd pathway to disrupt cellular homeostasis, whereas we over-expressed Foxo or Atg8 to balance it. Genetic perturbation of cellular homeostasis displays hallmarks of aging. Induction of Toll or Imd pathway locally and systemically leads to a decreased niche siz

SUBMITTER: Ghosh K 

PROVIDER: S-EPMC12536935 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature