Chromosome-specific oligo-painting provides insights into the cytogenetic basis of karyotypic stasis in paleo-allotetraploid <i>Cucurbita</i>.

ABSTRACT: Post-polyploid karyotype evolution represents a crucial cytological mechanism contributing to angiosperm diversification and speciation. Many polyploids show extensive karyotypic reshuffling relative to their pre-ancestors. However, karyotypic stasis is gaining popularity as an alternative evolutionary pathway following polyploidization, whose underlying cytological mechanisms remain poorly understood. Here, we successfully developed a set of enhanced oligo-painting (EOP) probes specific to 20 chromosomes of Cucurbita (2n = 40), a paleo-polyploid with very small chromosomes and rich genetic diversity. The probes generated robust fluorescence in situ hybridization (FISH) signals across six Cucurbita and one sister outgroup species. Cross-species EOP results confi

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC12537017 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature