Integrative multi-omics and causal inference reveal periodontal ligament cell-macrophage crosstalk under orthodontic force.

ABSTRACT:

Background

Orthodontic tooth movement requires synchronized mechanoadaptive and osteoimmune interactions, yet molecular pathways linking mechanical forces to immune-mediated bone remodeling remain incompletely characterized. This study aimed to elucidate molecular drivers and dissect cell crosstalk under orthodontic force, with a focus on their immunoregulatory consequences.

Methods

This study combined in vitro compressive loading of human periodontal ligament (PDL) cells with transcriptomic/proteomic profiling, and in vivo single-cell RNA sequencing of alveolar bone macrophages from murine orthodontic models. Mendelian randomization (MR) analysis leveraged genetic instruments from transcriptome-wide (n = 31,684), proteome-wide (n = 35,559), and immune trait genome-wide asso

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12537635 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature