CRISPR/Cas9- and Cas3-mediated modification of copy number variation in rice.

ABSTRACT:

Introduction

Copy number variation (CNV) is one of the crucial elements among genomic structural variations that span plant breeding. However, its impact on agricultural traits has remained elusive.

Methods

We modulated CNVs using two genome-editing technologies, CRISPR/Cas9 and Cas3, along with their verification methods in rice to elucidate the effect of CNVs and further harness to improve relevant agronomic traits.

Results

The addition of cytosine extension to the conventional single-guide RNA and its combination with Cas9 generated a frameshift mutation in parts of the OsGA20ox1 gene copies, substantially modifying its CNV. Phenotypes of the copy number variants revealed OsGA20ox1 copy number as a determinant of seedling vigor in rice. The Cas3 nucle

SUBMITTER: Park H 

PROVIDER: S-EPMC12537685 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature