Profiling extracellular vesicles from cerebrospinal fluid for classification of intradural spinal tumors.

ABSTRACT: Extracellular vesicles (EVs) transport biomolecules that could serve as biomarkers for disease diagnosis and monitoring. The clinical utility of EVs derived from cerebrospinal fluid (CSF) in patients with intradural spinal tumors (IST) has not yet been investigated. Here, we obtained EVs from CSF of adult patients with intraspinal ependymoma (n = 9), meningioma (n = 9), hemangioma (n = 4) and schwannian tumors (n = 7), as well as comparison group ('CG', normal pressure hydrocephalus, n = 7), by ultrafiltration. CSF-EVs were characterized by electron microscopy and nanoparticle tracking analysis. EV populations according to the presence of tetraspanins (CD9, CD63, CD81) were measured by imaging flow cytometry (IFCM). CD81⁺ EVs were more prevalent in the comparison group, meningio

SUBMITTER: Salviano-Silva A 

PROVIDER: S-EPMC12537865 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature