Formaldehyde vapour fixation enables multiscale phase-contrast imaging and histological validation of human-sized lungs.

ABSTRACT: Accurate diagnosis and characterization of lung disease increasingly rely on advanced imaging modalities capable of resolving fine microstructural details while minimizing radiation exposure. Phase-sensitive computed tomography (CT), particularly propagation-based imaging (PBI), offers superior soft tissue contrast but has historically been limited by the lack of compatible fixation techniques that preserve lung architecture post-excision. We present an adapted formaldehyde (FA) vapour fixation protocol designed to maintain human-sized lungs in a physiologically inflated and morphologically stable state. This approach prevents collapse of the delicate air-tissue interfaces, a major barrier to high-fidelity phase-contrast imaging and histological correlation. Our method enables high-resolut

SUBMITTER: Dullin C 

PROVIDER: S-EPMC12537873 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature