Near-isotropic super-resolution microscopy with axial interference speckle illumination.

ABSTRACT: Super-resolution microscopy has pushed the limits of biological imaging. However, achieving isotropic resolution across all spatial dimensions remains a challenge and often requires a complex and highly sensitive optical setup. Herein, we introduce axial interference speckle illumination-engineered structured illumination microscopy (AXIS-SIM), a minimal-modification approach that utilizes constructive interference from a simple back-reflecting mirror to enhance the axial resolution without additional phase control or complex beam shaping. AXIS-SIM provides superior optical sectioning and improves axial resolution beyond the typical axial resolution of conventional 3D-structured illumination microscopy (~300 nm), achieving lateral and axial resolutions of 108.5 and 140.1 nm, respectively.

SUBMITTER: Yoo H 

PROVIDER: S-EPMC12537971 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature