Shotgun haplotyping: a novel method for surveying allelic sequence variation.

ABSTRACT: Haplotypic sequences contain significantly more information than genotypes of genetic markers and are critical for studying disease association and genome evolution. Current methods for obtaining haplotypic sequences require the physical separation of alleles before sequencing, are time consuming and are not scaleable for large surveys of genetic variation. We have developed a novel method for acquiring haplotypic sequences from long PCR products using simple, high-throughput techniques. This method applies modified shotgun sequencing protocols to sequence both alleles concurrently, with read-pair information allowing the two alleles to be separated during sequence assembly. Although the haplotypic sequences can be assembled manually from the resultant data using pre-existing sequence asse

SUBMITTER: Lindsay SJ 

PROVIDER: S-EPMC1253838 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature