Ultrasonic-assisted enzymatic hydrolysis of Perinereis aibuhitensis using the RSM-BP-GA model.

ABSTRACT: The lack of ultrasound-assisted hydrolysis techniques often limits the efficacy of Perinereis aibuhitensis protein hydrolysate. Therefore, using P. aibuhitensis as the raw material and a 1:1 mix of neutral protease and flavour protease as the composite enzyme, we evaluated the degree of hydrolysis (DH). We systematically optimised the conditions for ultrasound-assisted enzymatic hydrolysis of P. aibuhitensis proteins using single-factor experiments, the response surface method, and a back-propagation neural network integrated with a genetic algorithm. The optimal hydrolysis conditions were as follows: a liquid-to-material ratio, 1:2.5; enzyme concentration, 970 U/g; ultrasound power, 390 W; sonication time, 31 min; enzymolysis temperature, 50 °C; ultrasound frequency, 30 kHz; pH, 7; and hy

SUBMITTER: Teng T 

PROVIDER: S-EPMC12538464 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature