Unspecific Peroxygenases for the Enzymatic Removal of Alkyl Protecting Groups in Organic Synthesis.

ABSTRACT: Selective protection and deprotection of hydroxyl groups is pivotal in multistep organic synthesis to circumvent undesired side reactions. Alkyl ethers are highly stable and atom-economic protecting groups (PGs), but demand harsh and hazardous conditions for removal, limiting their utility. Consequently, there is a high demand for biocatalysts as milder, selective, and scalable alternatives, which can be met by a class of heme-thiolate enzymes: unspecific peroxygenases (UPOs). Herein, we report the identification of UPO23 in a commercial enzyme panel as a robust biocatalyst for O-dealkylation reactions. UPO23 exhibited a broad substrate scope and efficiently removed methyl, ethyl, propyl, or allyl groups from protected primary, secondary, tertiary, and benzylic alcohols under ambien

SUBMITTER: Csechala LA 

PROVIDER: S-EPMC12538550 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature