Genetically Encoded Control of <i>In Vitro</i> Transcription-Translation Coupled DNA Replication.

ABSTRACT: The bottom-up reconstruction of cellular functions has gained increasing attention for studying biological complexity and for developing advanced biotechnological tools, including synthetic cells. A fundamental challenge is the ability to control and replicate DNA-encoded information within basic in vitro transcription-translation (IVTT) systems. Here, we constructed a transcription-translation coupled DNA replication (TTcDR) system that is based on a modified PURE (Protein synthesis Using Recombinant Elements) IVTT system and Φ29 DNA polymerase, which is controlled by external signals. To this end, we first established and characterized a PUREfrex 1.0-based TTcDR system. We then constructed and optimized TetR-based control of TTcDR activity, either by DN

SUBMITTER: Barthel S 

PROVIDER: S-EPMC12538582 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature