Development of a High Anthraquinone-Producing <i>Escherichia coli</i> Strain Using Malonyl-CoA Supply Pathway Engineering.

ABSTRACT: Anthraquinones are valuable compounds that are traditionally used as natural pigments and have diverse pharmacological activities, including antimicrobial and anticancer effects. In this study, we aimed to enhance the production of 1,3,5-trihydroxyanthraquinone (AQ256) using Escherichia coli (E. coli) as a host. AQ256 is biosynthesized from eight malonyl-CoA molecules via the type II polyketide synthase pathway. However, previous studies have reported very low production levels of AQ256 in E. coli (approximately 2.5 mg/L), mainly because of limited malonyl-CoA availability. To address this, we introduced a heterologous malonate assimilation pathway and reinforced the endogenous malonyl-CoA biosynthesis pathway. An E. coli strain harboring AQ256 biosynthetic gene

SUBMITTER: Suematsu T 

PROVIDER: S-EPMC12538586 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature