Outer Membrane Vesicles From <i>Bacteroides fragilis</i> Contain Coding and Non-Coding Small RNA Species That Modulate Inflammatory Signalling in Intestinal Epithelial Cells.

ABSTRACT: Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe-derived RNAs carried by outer membrane vesicles (OMVs) to host immune responses remains unclear. This study investigated the role of OMVs and OMV-associated small RNA (sRNA) species from pathogenic and commensal Bacteroides fragilis (ETBF and NTBF, respectively) in eliciting different immune responses from intestinal epithelial cells. To distinguish the differences in the sRNA profiles of the two strains and their OMVs, RNA-seq, qRT-PCR, and northern blotting were conducted to identify enrichment of discrete sRNA species in OMVs, which were also differentially expressed between the two strains. Specific

SUBMITTER: Sheikh A 

PROVIDER: S-EPMC12538813 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature