Comparative transcriptomics reveals differential carbohydrate and lipid metabolism in roots and nodules of Rhizobia-Inoculated alfalfa (Medicago sativa L.).

ABSTRACT:

Background

The symbiotic nitrogen-fixing system formed between alfalfa (Medicago sativa L.) and rhizobia requires precise regulation of carbohydrate and lipid metabolism to sustain their high-energy-demand system. However, metabolic divergence between roots and nodules remains poorly characterized.

Results

Using comparative transcriptomics, we analyzed gene expression profiles in pink nodules (PN), white nodules (WN), Pink nodule roots (PNR), white nodule roots (WNR), non-nodule roots (NNR) and control roots (CKR) from rhizobia-inoculated plants at 35 days post-inoculation. Key findings revealed metabolic specialization between tissues: PN exhibited elevated expression of lipid catabolism genes (MsECHIA, MsACX) and key genes of the TCA cycle regulators, driving direct energy

SUBMITTER: Lu BF 

PROVIDER: S-EPMC12538827 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature