Effect of monkeypox virus B14R protein fragments binding to IL-1β on apoptosis in HeLa cells.

ABSTRACT:

Background

Interleukin-1 beta (IL-1β) is a key mediator of antiviral immunity, and B14R proteins encoded by monkeypox virus (MPV) can interfere with its signaling. This study investigates the differences in IL-1β-binding capacity and apoptotic regulatory effects between two B14R protein isoforms encoded by MPV clades: the truncated B14R-180 (West African clade) and the full-length B14R-326 (Central African clade).

Methods

This study employed bioinformatics approaches to predict the binding affinity between B14R and IL-1β, and validated the findings through Enzyme-Linked Immunosorbent Assay (ELISA), flow cytometry, Quantitative RT-PCR (qRT-PCR), and Western blot analyses.

Results

the research demonstrates that B14R-180 exhibits superior binding efficiency to IL-1β comp

SUBMITTER: Zhu X 

PROVIDER: S-EPMC12538970 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature