Hydrogen sulfide induces regulatory B cells via glycolysis and mitochondrial ROS, attenuating LPS-induced lung injury.

ABSTRACT:

Background

Hydrogen sulfide (H₂S) is a gasotransmitter found in the human body that plays a crucial role in maintaining homeostasis of various biological systems, including the immune system. While prior studies have explored the effects of H₂S on T cells, its impact on B cells has yet to be elucidated.

Methods

We used sodium hydrosulfide (NaHS) as an exogenous donor for H₂S. LPS was used to activate B cells and promote their differentiation into plasma cells or regulatory B cells (B_regs). B_regs phenotypes were analyzed by flow cytometry and ELISA, and their immunosuppressive functions on neutrophils and CD4 T cells were assessed by co-culturing them with B cells. To investigate the therapeutic effect of B cells on acute

SUBMITTER: Jeong YS 

PROVIDER: S-EPMC12539000 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature