Mesenchymal stromal cells modulate neutrophil phenotype via paracrine signals.

ABSTRACT:

Background

Despite the recognized importance of neutrophils in cardiac repair following myocardial infarction (MI), their interaction with mesenchymal stromal cells (MSCs), particularly regarding polarization phenotypes and functional impacts, remains unclear. Here, we investigated these interactions across controlled in vitro systems and an in vivo MI model.

Methods

Human HL-60 cells were differentiated into neutrophil-like cells (dHL-60) and polarized toward N1/N2 states to test the MSC paracrine effects using indirect transwell coculture. Readouts included gene expression, cytokine profiling and functional assays. To increase translational relevance, indirect ex vivo cocultures of human MSCs with primary neutrophils isolated from MI mice or from MI patients were analysed

SUBMITTER: Cherry L 

PROVIDER: S-EPMC12539047 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature