Genotyping of Coxiella burnetii isolates in Iran using Multiple-locus variable-number tandem repeat analysis (MLVA).

ABSTRACT:

Introduction

Coxiella burnetii is the etiological agent of Q fever, a zoonotic infection recognized in various regions of Iran. Despite its significance, few studies have focused on determining C. burnetii genotypes in Iran. This study aimed to assess the genetic diversity of C. burnetii in Iran using the Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) method.

Methods

A total of 26 C. burnetii-positive specimens were selected from a repository of 119 specimens collected from various locations across Iran. These samples were previously identified as C. burnetii using nested PCR and TaqMan real-time PCR techniques. In this study, the MLVA method was utilized for genotyping C. burnetii isolates.

Results

Genotyping results from the 26 C. burnetii-positive sam

SUBMITTER: Dolatkhah F 

PROVIDER: S-EPMC12539059 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature