VESNA: an open-source tool for automated 3D vessel segmentation and network analysis.

ABSTRACT:

Background

Vasculature is an essential part of all tissues and organs and is involved in a wide range of different diseases. However, available software for blood vessel image analysis is often limited: Some only process two-dimensional data, others lack batch processing, putting a time burden on the user, while still others require tightly defined culturing methods and experimental conditions. This highlights the need for software that has the ability to batch process three-dimensional image data and requires few and simple experimental preparation steps.

Results

We present VESNA, a Fiji (ImageJ) macro for automated segmentation and skeletonization of three-dimensional fluorescence images, enabling quantitative vascular network analysis. It requires only basic experimental

SUBMITTER: Schuttler M 

PROVIDER: S-EPMC12539100 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature