The YAP1/GPX4 axis alleviates osteoporosis by affecting ferroptosis in osteoblasts.

ABSTRACT:

Background

Osteoporosis (OP) is a disease in which weak bones increase the risk of fracture. It has been reported that the occurrence of ferroptosis accelerated the progression of OP. However, the underlying mechanism of ferroptosis in OP remains unclear.

Methods

Clinical samples from OP patients were collected and ovariectomized (OVX)-induced mouse models with GPX4 knockout was established. The expression of genes and proteins was determined by RT-qPCR, western blot, IHC and IF. Bone mineral density (BMD) of the lumbar vertebrae was evaluated using DXA. Pearson correlation analysis was used to analyze the relationship between GPX4 expression and BMD. The femoral morphology was detected by HE staining. Images and relevant parameters of the femur were acquired using micro-CT.

SUBMITTER: Deng M 

PROVIDER: S-EPMC12539158 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature