Multiplex long-amplicon sequencing for comprehensive molecular surveillance of Plasmodium falciparum resistance to artemisinin and partner drugs in artemisinin-based combination therapies (ACTs).

ABSTRACT:

Background

Antimalarial resistance, especially artemisinin resistance, in Plasmodium falciparum threatens global malaria control. Molecular surveillance of Plasmodium falciparum drug resistance is critical for monitoring the efficacy of artemisinin-based combination therapies (ACTs). Current molecular surveillance tools for Plasmodium falciparum artemisinin resistance are restricted to predefined polymorphism hotspots in limited loci (Pfk13 domain), failing to capture novel mutations in emerging resistance genes (Pfcoronin, Pfubp1, Pfap2μ) or cover complete coding regions. This study addresses this gap by developing an optimized long-amplicon panel for comprehensive, full-gene resistance surveillance.

Methods

Six genes were selected for the multiplex PCR amplification, inclu

SUBMITTER: Li F 

PROVIDER: S-EPMC12539163 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature