3D imaging-driven assembly of multispecies biofilms with antagonistic activity against undesirable bacteria.

ABSTRACT: Engineered synthetic microbial communities (SynComs) forming biofilms with antagonistic activity offer a promising strategy in biotechnology to prevent harmful bacterial settlement and reduce reliance on chemical antimicrobials. However, strain selection criteria and antagonistic mechanisms remain unclear. This study presents a bottom-up approach integrating 3D fluorescence imaging with high-throughput analysis of multistrain biofilms. Our findings reveal that competitive strains against undesirable bacteria may also exclude desirable community members, highlighting the need for compatibility control in SynCom assembly. SynComs composed of Bacillus velezensis and Pediococcus spp. enhanced pathogen exclusion compared to single strains. Temporal analysis of biofilm interactions

SUBMITTER: Gueneau V 

PROVIDER: S-EPMC12539569 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature