Condensation of the RNA chaperone Hfq is coupled to inhibition of glucose uptake and contributes to the stabilization of regulatory RNAs in nitrogen-starved Escherichia coli.

ABSTRACT: Ribonucleoprotein condensates are membraneless compartments that concentrate RNA-binding proteins and RNA, and play key roles in cellular adaptation across both eukaryotes and bacteria. While the biological roles of ribonucleoprotein condensates are better understood in eukaryotic systems, the knowledge of metabolic processes that govern their formation and their contribution to stress adaptation remains at a nascent stage in bacterial RNA biology. Hfq is an RNA chaperone conserved in many bacteria that undergoes condensation in response to diverse stresses. Using nitrogen (N) starvation in Escherichia coli as a model stress condition, we show that Hfq condensation occurs independently of any extracellular metabolic cues, cytoplasmic shrinkage that cells undergo during N starvation, or the

SUBMITTER: McQuail J 

PROVIDER: S-EPMC12539622 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature