The knock-in atlas: a web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines.

ABSTRACT: Various cell engineering techniques have been developed by leveraging the CRISPR-Cas9 technology, but large-scale resources for targeted gene knock-in are still limited. Here we introduce the Knock-in Atlas, a web resource for gene tagging by fluorescent proteins by inserting artificial exons in target gene introns. To produce knock-in cells efficiently and reproducibly, we carefully chose and catalogued guide RNAs (gRNAs) for targeting genes in the human and mouse genomes by taking the gRNA efficacy scores and protein structures around the insertion sites into account. As of August 2025, we have characterized knock-in cell lines for 350 proteins, with a focus on RNA binding proteins, by flow cytometry and confocal microscopy. The transfection and flow cytometry protocols were optimized fo

SUBMITTER: Hanai Y 

PROVIDER: S-EPMC12539675 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature