Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.

ABSTRACT: Walnut (Juglans regia L.) is an economically valuable tree species globally, renowned for its nutritious nuts and quality timber. However, walnut breeding is significantly constrained by inherent biological factors, and an efficient and reliable genome-editing system has yet to be established. In this study, we developed an optimized walnut genome-editing platform by systematically selecting superior receptor from 30 walnut cultivars using genotype-dependent direct somatic embryogenesis and regeneration systems. Walnut cultivar HT-14 exhibited the highest embryogenic induction (53.33%) and regeneration efficiency (85.33%), and 35S: RUBY was effectively expressed in somatic embryos of the HT-14 genotype, proving it ideal receptor material for genetic transformation. Additionally, 12

SUBMITTER: Song L 

PROVIDER: S-EPMC12539868 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature