Strand-specific quantification of 8-oxo-dG and apurinic sites via Ligation-Dependent Probe Amplification (LPA).

ABSTRACT: Oxidative DNA damage, characterized by the prominent lesion 8-oxo-7,8-dihydroguanine (8-oxo-dG), is linked to mutagenesis and genome instability. Accurately mapping these lesions with strand specificity and high resolution remains a major challenge, limiting our understanding of damage dynamics during transcription and repair. Here, we introduce a novel, highly sensitive ligation-dependent probe amplification (LPA) method that enables quantitative, strand-specific analysis of 8-oxo-dG and apurinic (AP) sites at single-nucleotide resolution. Our technique uses enzymatic digestion, highly selective ligation, and quantitative PCR to distinguish damaged from intact DNA strands, offering detailed insight into lesion localization and repair kinetics. When applied to estrogen-stimulated breast ca

SUBMITTER: Romano A 

PROVIDER: S-EPMC12540035 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature