Integrating macro-microscopy, DNA barcoding and HPTLC for quality assessment of berberine containing botanicals traded as Maramanjal/Daruharidra.

ABSTRACT:

Background

Daruharidra/Maramanjal is one of the most popular shrub used in Ayurveda, Siddha and other Indian medicinal systems. More than one botanical source is traded under this name, predominantly Berberis aristata and Coscinium fenestratum with an annual trade of 1000-2000 metric tonnes. The herbal drug trade is often reported with misidentification, adulteration and/or substitution issues due to morphological resemblance and confusion in vernacular names. This work aimed to integrate macro-microscopic, DNA marker strategies and phytochemical assay to differentiate Berberis aristata from its traded sources.

Material and methods

Thirteen marketed samples and one authentic field sample from natural habitat were collected from various regions of the Indian market under the

SUBMITTER: Koppala Narayana SK 

PROVIDER: S-EPMC12540061 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature