Optimizing monocyte-derived immune cell cultures: comparing xeno-free and xenogeneic conditions.

ABSTRACT: Culture conditions significantly affect the phenotype of immune cells. This study compared, for the first time, the impact of xeno-free human AB serum and xenogeneic fetal bovine serum (FBS) culture conditions on the surface marker expression of monocyte-derived macrophages (Mo-Mø) and dendritic cells (Mo-DC) using spectral flow cytometry. Monocytes were differentiated into macrophages or Mo-DC over 6 days. M0 macrophages were polarized toward M1-like or M2-like macrophages, and Mo-DC were activated using LPS. Differentiation was successful in both conditions. Despite cells exhibiting autofluorescence, distinct phenotypes based on selected differentiation markers were observed. CD1a expression was lacking in AB cultures, while expression of CD16, CD163 and the co-stimulatory molecules CD80

SUBMITTER: Marek N 

PROVIDER: S-EPMC12540148 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature