A Mutation-Based Reverse Vaccinology Approach Considering Variability in Epitopes to Combat Multi-Strains: A Study Using Glycoprotein of LASV.

ABSTRACT: Lassa virus (LASV) remains a persistent threat to public health, and to combat this, various therapeutics have been developed, but their effectiveness is limited due to the virus's strain variability. Therefore, mutation-based reverse vaccinology approaches were implemented to formulate an epitope-based vaccine against the LASV, considering the variability in the glycoprotein. The glycoprotein was examined to screen out the B and T cell epitopes and further examined for the immunodominant epitope activity assessment. These epitopes were mapped with the identified position to introduce variability. 2 LBL (Linear B-cell lymphocyte), 21 MHC-I (Major Histocompatibility Complex Class I), and 8 MHC-II potential epitopes were considered for wild and mutated (based on the mutation mapping). The wi

SUBMITTER: Mishra SK 

PROVIDER: S-EPMC12540218 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature