Overproduction of β-barrel outer membrane proteins in <i>Escherichia coli</i> BL21(DE3) induces hypervesiculation.

ABSTRACT: Aim: Gram-negative bacteria release outer membrane vesicles (OMVs) that fulfill many functions including survival during stress conditions, delivery of virulence factors, and nutrient acquisition. Additionally, they are increasingly used as an alternative for live bacteria in vaccine development and as a platform for bioengineering. Recently, OMVs have also been applied to express recombinant outer membrane proteins (OMPs) in their natural context as an alternative to the cumbersome reconstitution in liposomes. Here, we use an Escherichia coli strain that lacks four major OMPs for selective expression of the β-barrel assembly machinery (BAM) complex and PhoE in OMVs. Methods: OMV production of Escherichia coli BL21(DE3) and its omp8 derivative upon overexpressio

SUBMITTER: Sahu S 

PROVIDER: S-EPMC12540271 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature