Multi-omics analysis reveals Angelica dahurica radix extract alleviates migraine in rats via gut microbiota-metabolome-gut-brain axis regulation.

ABSTRACT:

Background

Emerging evidence links gut-brain axis dysregulation to migraine pathogenesis. Angelica dahurica Radix (Baizhi) demonstrates clinical efficacy in migraine management, yet its mechanisms involving gut microbiota-metabolite crosstalk remain unelucidated.

Methods

A nitroglycerin (NTG)-induced migraine rat model was established. Migraine behaviors were video-recorded. Enzyme-linked immunosorbent assay (ELISA) was used to quantify neuroinflammatory and neurotransmitter markers (5-HT, CGRP1, TNF-α, NO, PGE2, DA) in plasma and brain tissue. Intestinal barrier integrity was evaluated by immunohistochemical staining of tight junction proteins (Occludin/ZO-1) and hematoxylin-eosin (H&E) staining of colonic tissue. Gut microbiota composition was analyzed via 16S rRNA

SUBMITTER: Lian Y 

PROVIDER: S-EPMC12540402 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature