The genetic and proliferation characterization analysis of novel coxsackievirus A12 in Beijing, China.

ABSTRACT:

Introduction

Coxsackievirus A12 (CVA12) is a serotype of Enterovirus A. Its evolutionary and molecular characteristics remain poorly understood.

Methods

The metagenomic Next-Generation Sequencing (mNGS) strategy were used to investigate the viral diversity. The viral isolation, proliferation assays, phylogenetic relationships and recombination events were analyzed.

Results

In this study, nine clinical specimens collected in Beijing, China, during March 2010 to October 2019 were identified as CVA12 positive, among which five were confirmed by mNGS. Then five CVA12 strains were isolated, and the proliferation assays demonstrated the preferential replication of CVA12 in rhabdomyosarcoma (RD) cells, with rapid intracellular replication before being released extracellularl

SUBMITTER: Han Z 

PROVIDER: S-EPMC12540438 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature