Spermidine improves seed viability in <i>Allium mongolicum</i> by regulating AmCS-mediated metabolic and antioxidant networks.

ABSTRACT:

Introduction

Seed deterioration involves oxidative damage and disrupted energy metabolism, yet the genetic mechanisms underlying aging resistance in Allium mongolicum remain unclear.

Methods

In this study, seeds primed with 0.8 mM spermidine (Spd) and stored for varying durations were subjected to transcriptome sequencing, targeted energy metabolite profiling, and assessments of antioxidant systems and energy metabolism enzymes.

Results

We identified citrate synthase (AmCS) as a pivotal candidate gene involved in delaying aging processes. Under standard growth conditions, AmCS-overexpressing Arabidopsis lines exhibited a 15.55% higher germination rate compared to wild-type (WT), with enhanced activities of superoxide dismutase (SOD) and per

SUBMITTER: Men Y 

PROVIDER: S-EPMC12540469 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature